Country, rock

There are many genres in today's music for one to choose. Many of them are not so different, for example, Country and Bluegrass. Then again, some genres are completely different like Metal and Polka. There are many complex conversations and arguments over which genre is closest and farthest apart. In my opinion the hardest compare and contrast of them all would be between Country and Rock. Firstly, some may wonder why Country and Rock sound so similar.Both of the genres come from the same musical roots. They come from the early nineteen hundreds genre known as Blue's music. They both have similarities in their musical atmosphere. For example, Jason Alden's country song she's country has a heavier rift and a slower, drop tuned beat for the average country song. On the other hand, Kings of Leon, a rock band, have a faster upbeat tempo, and a more acoustic feel for a typical rock band. For example, their song Back Down South has a southern, good ole boy country sound.There are also ways that the two types of music sound different. For instance, Slipknot's feel is a more brutal, head banging, and violent sounding. As in their song Psychosocial, where the guitars are shredding and the drums sound as if hell is aging over. In contrast to that you have the country band Brooks and Dunn, whose songs usually have more of a down home, boot tapping harmony. For example, the song Boot Scoot Bogey in which has southern, mellow party melody.Secondly, they are alike, but yet very different in the concept of how they dress and display their image. You might see someone in a cowboy hat and torn Jeans, or you might see someone with long, dark hair and a black t-shirt. Most country bands prefer cowboy hats, dress shirts, tight blue Jeans, and cowboy boots. The lead singer of an average entry band usually has short, stylish hair with a clean beard. They are usually better groomed. But lead singers in a rock band have tattoos, piercing, long hair and facial and more drinking and dru gs.The goal of academic writing is to inform an audience about a particular topic in a very professional manner. Effective academic journal writing does involve clearly stating your purpose and presenting your information in a manner that is simplistic enough for others to read and understand while still having the tone of an academic piece. Tone is a literary technique that is a part of composition, which encompasses the attitudes toward the subject and toward the audience implied in a literary work.The tone of the memo and any kind of paper depends entirely on the audience. Academic writing should be concise, without being completely stripped of the necessary facts that are needed to support your topic. The content impact is how your reader and listeners' feel about the subject at the end of your writing. They should be able to follow your story easily, it should flow together like music, the story should inform them of the subject, and should not leave them with questions at the end.

Black Asthetics and Toni Morrison

The black arts, or the black aesthetic, movement was born among the black artist as a response to the ideologies of the black power in the 1960’s. The movement was a continuation of the 1920’s and 1930’s Harlem Renaissance that had begun the tradititon of rediscovering the roots os black culture and heritage,dating back to slavery. Some of the major literary figures of the Harlem era included authors James Weldon Johnson, Langston Hughes and Nella Larsen. The Black arts emerged to promote art that illustrated African-American music, languages, heritage, and beauty. In order to be substantial, art had to have a proudly black subject matter and style; be it sculpture, a piece of music, a novel or a poem. Empowered by the concepts of the black power, the movement inspired the emergence of the black theatre groups, magazines, and printing presses. Literature influenced by the black arts concepts struggled to abandon W. E. B. Du Bois’ idea of double consciousness, which meant blacks were constantly struggling towards the white culture’s ideals, even though the dominant society disabled them for reaching the Eurocentric goals. Mirroring themselves against the value structure of the oppressive white society was depriving the blacks of their empowerment. Black writers wanted to concentrate on solving the problems of the African-American community from the inside, developing awareness of the rich black heritage and gearing the co mmunity to realize it worth. The Black Arts movement brought the time for blacks to stop internalizing the image of being the inferior in the society as a whole. The black population had to find strength, beauty and self esteem within the black community. The black arts, characterized by acute awareness, produced writers like Toni Morrison, Ishmael Reed, and Alice Walker. Toni Morrison undeniably is an author who internalizes the main concerns of the black aesthetic. She writes about black oppression, consciousness and tradition. Her major characters’ are black and they are in constant search for their ethnic identity. The first African American writer to win the Nobel Prize for literature in 1993, Toni Morrison is a leading voice in current debates about the construction of race and black marginality in literature and culture. As a prominent writer of the age she refuses to allow race to be marginalized in literary discourse. Throughout her writing Morrison uses narrative forms to express African Americans' dislocated, oral tradition, and culture, and reclaim African American's historical experiences. She profoundly uses the fictive narratives to transfigure the old south; the bedrock of black dehumanization, degradation and sorrow into an archetypal black homeland, a cultural womb that lays claim to history's orphaned, defamed and disclaimed African children. In her novels Morrison humanizes black characters in fictions that strive to overcome and excavate enforced invisibility of African Americans' social reality. Morrison critiques the mainstream thinking and acclaims that black writers and black characters are the relative means by which text demonstrates to be human and superior. Imagination is possible in the presence of black characters and black contents. At the same time talking African discourse is inferior and submissive tends to impoverish cultural interpretation of reality. Morrison questions the validity and vulnerability of a set of assumptions conventionally accepted and taken for granted among literary historians and critics. Africanist presence, in a constitutive part in the entire history has been rejected. Morrison in Playing in the Dark: Whiteness and Literary Imagination proposes, â€Å"[t] he contemplating of this black presence in central to any understanding of our national literature and should not be permitted to hover at the margins of the literary imagination† (5). Morrison argues that American culture is built on, and is premised by, and always includes, the presence if blacks', as slaves, as outsiders. She likens the unwillingness of academics in a racist society to see the place of Africanism in literature and to the centuries of unwillingness to see a favorite discourse, concerns and identity. She posits whiteness as the ‘Other' of blackness, a dialectical pair, each term both creates and excludes the other: no freedom without slavery, no white without black. The major themes of Toni Morrison's writing is to redefine the notion of white American canonical texts and their idea of African American writing as being non-canonical or inferior. She demonstrates the idea of racial superiority and hegemonic culture in her writings. Morrison, in the preface of her critical work Playing in the Dark: Whiteness and Literary Imagination says she is â€Å"struggling with and through a language that can powerfully evoke and enforce hidden signs of racial superiority, cultural hegemony and dismissive ‘Othering' of people and language which by no means marginal or already and completely known and knowable in my work† (XI). It is clear that Morrison's writing is different from that of mainstream white discourse, which always bserves that African American literature is subsidiary product. Her intention, thorough her writing , is to reinterpret and redefine the hidden, dislocated and alienated Afro-American presence in American mainstream discourse and claim that Afro-Americans are no more inferior human beings. Toni Morrison's fiction demonstrates a central interest in the issues of boundary, attachment, and separation. Her characters experience themselves as wounded, or imprisoned by racial and economic divisions within American culture. The boundaries that circumscribe black people are not only the prejudices and restrictions that bar their entry into the mainstream but the psychological ones they internalize as they develop in a social structure that historically has excluded them. Toni Morrison draws from a rich store of black oral tradition as well as from her own imaginative angle of vision to illuminate the potentialities for both annihilation and transcendence within black experience. Black lore, black music, black language and all the myths and rituals of black culture are the most prominent elements in Toni Morrison's writing. She feels a strong connection to ancestors because they were the culture bearers. She thinks that it is the responsibility of African American writers to dig out that annihilated history and secure the importance of it in the making of American civilization. Toni Morrison ranks among the most highly regarded and widely read fiction writers and cultural critics in America. As a critic she refuses to allow race to be relegated to the margins of literary discourse. She focuses on the importance of African American's oral and musical culture and to reclaim black historical experiences. Morrison says that African American have rediscovered texts that have long been suppressed or ignored, have sought to make places for African American writing within the canon, and have developed ways of interpreting these works.Works CitedMorrison, Toni.  Playing in the Dark: Whiteness and the Literary Imagination. Cambridge, MA: Harvard UP, 1992. Print â€Å"Toni Morrison.†Ã‚  Wikipedia, the Free Encyclopedia. 21 May 2011. Web. 23 May 2011. .Welcome to Black Aesthetics Institute. Web. 23 May 2011. .

Answer 2 questions Assignment Example | Topics and Well Written Essays - 250 words - 2

Answer 2 questions - Assignment Example In United States, the family decline is apparent due to reasons such as increased rates of illegitimacy, unmarried couple of households and divorce. In US, there has been an extensive increase in the rate of illegitimacy. The birth rate of unmarried women has gone up by a significant percentage. Research showed that the number of illegitimate babies is going up day by day as well increasing the number of single parents. Family divorce is another reason that has tremendously led to family declines (Hobson 76). The family decline is a major has become more obvious in the United States. Parent around the world experiences joys and heartaches due to rewards and challenges of becoming parents. Parenting has remained a baffling subject where everyone has an opinion, but most people do not agree. Parents have a responsibility of preparing children for economic, physical, and psychological situations in which the children must thrive and survive. Being a financial provider is not a choice but a demanding responsibility for parents. Parents often find themselves in a tag of war between the works demands and finding time for their families. Mostly, parents sacrifice their family time for their work by means to ensure that they keep their jobs (Hobson 89). Some parents make a balance between the responsibilities as financial responsibilities and parents as they consider the two responsibilities intertwined. It is quite possible to fulfill the role of a financial provider and a role of a parent at the same time. Maintaining the two responsibilities is realistic as the two r esponsibilities are equally important. Parents believe that fulfilling their role, as the good provider is a fine balance between the families. Parents adjust their work shifts so that they can have time with their children and

MGT506 - Strategic Leadership, Mod 2 SLP Essay Example | Topics and Well Written Essays - 750 words

MGT506 - Strategic Leadership, Mod 2 SLP - Essay Example According to the definition, open systems elaborate an organizational model that allows the organization to interact with the society i.e. take necessary input and deliver outputs. Considering that factor, open social systems and organizations have a tendency to adapt to social changes according to feedback provided and makes required changes internally. Hence, open system of an organization is a live mechanism that is continuously evolving and showing changes in its structure and behavior. In contrast with an open system, closed system shows a deterministic approach. This system operates in the absence of feedback loop that is detached from external factor. It can be said that closed systems allow its components to remain within the sphere of their identity that shows a constant character. In the light of these doctrines, it can be established that language and society are closed mechanisms that only shows slow alterations only when the system needs external information and shows gr adual changes over decades and sometimes centuries. Similarly, external discharge of information is also selected and limited (Katz & Kahn, 1966). It has been observed that Martin Luther King was a transformational leader who used pathos, ethos and symbols for influencing his followers. It can be observed that MLK has emerged as the leader of African Americans due to consistency in his behavior and lack of adherence to requirements of other social forces. This astute and consistent behavior makes him a follower of closed system regime. These demands had a concrete background based on bias shown towards Blacks by American society that spreads on the span of centuries. Basing his requirements on the discriminated behavior, he demanded equal rights for African Americans from the beginning and did not alter his requirements in response to other social factors. Requirement was only one which entails that African Americans citizens must have equal legal and ethical status as the white one s (Luther, 1963). On the basis of this stature, he managed to illustrate a behavior of constant struggle and fundamentality in his principles. With the help of this consistency in overall behavior, he managed to attract and retain millions of Blacks as his followers which ended up brining revolution in American society and legislation. In comparison with MLK, Muhammad Ali Jinnah was a follower of open-system ideology. Jinnah himself was a learned barrister who had spent most of his life in England. He was a follower of a belief that Muslims and Hindus in subcontinent should live together. However, after spending considerable time in All India Congress, he observed the bias against Muslims that made him lay down the foundation of Muslim League in Subcontinent, the only Muslim party that legalized the initiatives for separate country for Muslims (Wolpert, 2005). Where Jinnah initially worked on transforming the legal and ethical status of Muslims in subcontinent like MLK, his percepti on changed over time on the basis of events that lead him to believe that Muslims need to have a separate homeland for earning a right of respectful living. Therefore, after late 1920s, the direction of

Macroeconomics Assignment Example | Topics and Well Written Essays - 1000 words

Macroeconomics - Assignment Example The amount of trade deficit is 2008 is (422,905 - 460,665) = -37760. The amount of trade deficit is 2009 is (422,905 - 460,665) = -32023. iv. The value of real GDP in 2009 is (Nominal GDP of 2009/GDP deflator of 2009) = ? (1,373,636/ 101.4) million = ?13546.71 million. v. The current macroeconomic situation of the UK can be defined as economic recession. So, there has been no surprise that the economy has experienced a negative rate of growth form 2008 to 2009. The fall in consumption spending, investment, and net exports (exports minus imports) can be explained by the prevalence of ongoing economic recession which cause fall in demand. The rise in government expenditure can also be explained by the current macroeconomic situation. In order to deal with the recessionary situation, the government has undertaken certain measures to boost aggregate demand of the country. One of these corrective policies is increased government spending that has been reflected by increased government exp enditure form 2008 to 2009. 2. There is some problem with this question†¦ What are the coefficient of r in the expression for investment and the expression for real money demand? The coefficients are important as they denote the rate of response to change in interest rate. if there is no coefficient it should be assumed that coefficients are equal to 1. But this case is either impossible or very rare!!!! 3. The main forms of unemployment are as follows: Structural unemployment: Structural unemployment is a particular form of unemployment. This kind of unemployment takes place when a labor market becomes unable to offer jobs to everybody who wants to work due to the fact that the level and type of skills of unemployed individuals do not match with the skills required for the jobs available in the market. Structural unemployment usually lasts very long. Very often technological unemployment which results from substitution of workers by machines might be recognized as structural u nemployment. In order to deal with structural unemployment, there should be an increase in aggregate demand of the economy. If there is a fall in aggregate demand, then many individuals become unemployed and enter into the vicious circle of poverty, while their skills become obsolete. Since the present economic scenario of the UK can be characterized as economic recession, structural unemployment should be there in the economy as production has reduced drastically and the UK economy has failed to provide job to all who wish to work. Frictional Unemployment: Frictional unemployment takes place during the time period when a worker shifts one job to another. In other words, frictional unemployment takes place during transitioning from one job to the other. Very often, frictional unemployment is referred to as search unemployment. Very often this unemployment is voluntary in nature. Frictional unemployment can always be found in an economy. Thus in UK economy, it would be quite easy to find out frictional unemployment. Cyclical Unemployment: Cyclical unemployment is very often referred to as Keynesian unemployment. It is also known as deficient-demand unemployment. Cyclical unemployment takes place when there is scarcity of aggregate demand for providing jobs to everybody who are willing to work. When demands for goods and services reduces, the level

MICROECONOMICS Essay Example | Topics and Well Written Essays - 1750 words

MICROECONOMICS - Essay Example It must be noted here that for the private firms, the altitude of the supply curve from the horizontal axis determines the minimum price (average) at which a specific quantity must be sold so that supplier would be able to cover the variable cost. He cannot afford to sell at any less value. It is vital to understand the meaning of demand and supply as it has been used in the micro economics models. It must be perceived as the flow of inventory, inwards for supply and outwards for demand. Demand and supply do not have a direct link between them but it is the market that decides their levels. Finding the equilibrium price A unique price exists in the market that would establish perfect equilibrium between demand and supply known as equilibrium price. This price is a great leveller for both buyers and suppliers and there is least scope for manipulation of prices driven by greed in a long run. In a perfect market, there is an equilibrium quantity that exists for the equilibrium price and either way shifts in the value of this equilibrium due to misallocation of resources (whether too few scarce resources are being used or too many scarce resources are being used) would result in dead- weight loss. ... It is more or less automatic. Trouble in the public sector This is in complete contrast to what happens in the public sector. When it comes to public services which are run by governments, there is hardly any interaction of demand and supply curve as there is absence of ‘feedback’ factor. So there is no automatic adjustment of the prices as government rely on taxes for covering the cost that is incurred in rendering these services and consumers hardly have any choice. This is why misallocation of resources is a commonplace under government led enterprises and it would be better if governments restrain themselves from getting engaged into non vital activities. Whenever government tries to control the price either by deciding the floor price or the upper cap, it runs the risk of misallocation of resources. It would result in ‘dead - weight losses and could get worse. Analysing the effects of minimum wage law indicates that it fails to achieve the objectives which it was originally devised for. It increases the supply of the unskilled labour in the market and at the same time decreasing their demand. It fails to serve the poor, worsens the problem of unemployment in the long run and increases the burden over the taxpayer in an unjustified manner. Even the ‘earned income tax credit’ program has the similar consequences though it is not as bad as minimum wage law. Under both the schemes, government seems to address the symptoms and not the root causes of the problem. It is relevant to suggest here that instead of attempting to control the prices directly, it would be much better to influence the market. Policies must be such that they help

The Merits of Privatization Research Paper Example | Topics and Well Written Essays - 500 words

The Merits of Privatization - Research Paper Example tes, transcontinental railroad constructed by public and private partnerships during the nineteenth century is one of the earliest examples of privatization in the US. The transcontinental public private partnership is always cited as an example by the people who support privatization. Privatization not only brings efficiency but it also helps in curtailing costs and reduces bureaucracy. (Loulakis, 2003) The recent financial downturn and government bailouts have severely affected the American economy. With the government deficits and costs of borrowing increasing day by day, many leading economists have advocated privatization of government functions which would bring budgetary discipline and would reduce government’s financial burden. Though the consumers are greatly benefited by the reduction in the costs of goods and services due to privatization, the only point which may negatively affect the citizens is accountability. Citizens do not have any control over private companies and cannot punish them, like they can punish the government by not voting for them in state or central elections. Another point of concern with privatization is that private firms operate with only one motive that is profitability, which is secondary in government owned and operated firms. Though, privatization has greatly improved product quality and cost, there are certain services which should not be privatized. Sectors like defense, law enforcement, education and basic healthcare should always be under State control. The future of the country depends a lot on the above mentioned sectors and therefore, the motive of cost cutting, profitability and competition should not come into consideration. However, certain functions like sourcing of certain products can be outsourced. Another reason why these sectors should be under State control is because in case of any adverse events the government must have the capacity and the control to handle the situation quickly and

Important aspects in the development of any construction Essay

Important aspects in the development of any construction - Essay Example Important aspects in the development of any construction Designing, planning and constructions are important aspects in the development of any building, highways, airports, dams and railroads. Therefore, it is significant to first of all, clearly design, plan and construct what needs to be achieved and accomplished and then start working on the construction. If the designing and planning phase are not managed appropriately, then it could significantly hurt and damage the outcomes of the project. Moreover, such situations could also increase the cost, outcome and time of the project (Chan, Scott, & Chan, 2004). This has been one of the reasons why the project managers emphasize a lot on the initial designing and planning stage before moving to the actual construction phase of the project. This report presents the case of a client that owns a two-storey building. The client wants to increase the available area of the building and therefore, he wants to add three more storeys to the same building. For this purpose, this report presents the conceptual design of adding three more storeys to the building. At the time of planning, designing and construction, there are some considerations that have been kept in mind. For instance, one of the major considerations is that the site is suited in open countryside and as a result, there will be high wind as well with an approximate speed of 1 kN/m2.

Analytical Essay Example | Topics and Well Written Essays - 2000 words - 1

Analytical - Essay Example Is media, too, entitled to change its view from ethical to business oriented? While, we cannot take the huge task of judging the media across the world, we will be focusing on the Australian media scenario, learning form the facts, analyzing a few media channels and coming to a conclusion – Is the contemporary Australian media following in the wheels of entertainment more than education? While the question, indeed, is big, the discovery towards the conclusion will prove to be startling nevertheless. One of the presets of any education in media explains its students that the first and foremost task and responsibility of media towards the viewers is to â€Å"inform.† However, are these precepts only a part of the necessary and age old course material, or do these young students really do follow these in real lives becomes of question. Several nations have seen a change in their media trends. Australia, too, cannot ignore the changes that have come about in the media scenario. Be it broadcast or the print media, the changes that have come about are inevitable. However, are these changes absolutely necessary, is the big question. Several researches have been conducted and several journals and news stations monitored. The results of all have come to the same thing – the Australian media is, indeed, giving in to the pressure of the monies and becoming increasing more business oriented than any other time earlier. While we have explained that media is changing the scenario, how is it that we reached the conclusion? The same is cited here. The Australian media is enjoying what is called as the complete â€Å"editorial freedom and freedom of speech and expression† like no other media in any democracy across the world. Despite the freedom that the journalists and media exercise, the journalists are often blamed by the citizens and masses in general that they abuse the power that they have been asserted with. Even when the Princess Diana

Social Media Essay Example for Free

Social Media Essay Social media today is one of the most advanced platforms for companies to market their product instantaneously and to a large audience with just a click of a button. With the current technological boon the world is undergoing, social media has definitely taken the front seat and helped an array of organisations from different fields to become some of the world’s leading profitable organisations. Social media marketing (SMM) is a form of Internet marketing that utilizes social networking websites such as Facebook, twitter, instagram etc. as a marketing tool to advertise and market their products to the general public. This essay will focus on the different uses that social media websites have to cater to marketing with reference to a multinational, e-business company called amazon. Since the internet is such a large market with a colossal number of social networking sites, it is important for organisations to plan before they venture into a marketing campaign on what exactly they need to achieve.. see more:social networks and our young generation. speech Amazon has now dominated the world of e commerce and online retailing with an immense competitive advantage by engaging customer satisfaction and planning a genuine marketing strategy. A large proportion of amazon’s marketing is on social media websites which is an appropriate place to find a large target audience to its website. Some of the companies’ key motives and strategy on social media marketing are discussed below. Firstly, amazon has established a clear cut goal of marketing online to increase brand awareness by promoting and advertising with sales offer to its target group on social networking arenas. Secondly the company has figured on what types of social media platforms they need to market their website on. Some of its primary marketing campaign takes place on sites such as Facebook, YouTube, twitter and Instagram, which are largely driven and operated by a young population who are more likely to shop online. Also its has engulfed and adapted strategies such as engage potential customers, prioritize the brand, measure the outcome, listen to feedback and control and develop its image. Social media has gained so much attention today, with a large population hooked onto social networking sites. This has given a massive opportunity for entities to market their products on these sites to a large audience with  sometimes at no cost to post online. amazon is a pioneer in social media marketing with its active involvement in advertising and promoting its business online. from directly emailing existing customers about sale offers to posting about its products on their facebook fanpage, it definitely has a large and active social media marketing involvement.

Advantages and Disadvantages of Cryopreservation

Advantages and Disadvantages of Cryopreservation In the world of science this two procedures Vitrification and Slow cooling are use to keep the biological materials such as cells, bone marrow , DNA etc at the low temperature , when compared to their normal temperatures. These two procedures will come under the Cryobiology. Cryobiology: It is the study of life below the low temperature. Background: In the centuries 2500 BC this was used by the people of Egypt for the medical purpose. They used to stop the bleeding and injuries during the injuries. In the latter centuries this was brought into popular by Robert Boyle. For the first time it was the Christopher Polge who used the bull sperm in cryopreservation. The 1970s brought great development in cryobiology by Zeo Layland who brought Slow Cooling technique which laid a path to the birth of first human embryo frozen, which latter used all over the world for the animals, cells and human biology. In the year 1986 Dr. Christopher Chen in Australia used the slow frozen oocytes for the pregnancy in the world for the first time. Advantages of cryobiology: Helps in the preservation of biological materials. By this the biological materials can be preserved for long time. Sperm, gametes, embryos, tissues, bone marrow, organ can be preserved. Helps to study the adapting nature of plants and animals under the low temperature. Cryopreservation: This is the process, which come under the Cryobiology. This is the process in which the cell is kept under the very low temperature which causes the cell to stop its biological chemical reactions and finally the cell leads to death. But sometimes the cell which is kept under the process of cryopreservation may get damage, when it is taken to the low temperature. Some of the biological materials are kept under very low temperature which is the liquid phase of the liquid nitrogen. Because it is the best process for the preservation some complex biological compounds which lead to stop their biological chemical reactions. In order to be free from the risk the most two techniques used are the Slow Cooling and Vitrification. Background: James Lovelock is the important person who made the Gaia theory fame. Using this theory he said that the damage that occurs to the red blood cells is due to the osmotic stress during the process of the freezing. In the early years of 1950s he said that when the cell faces the increase of salt concentration make it to dehydrate for the loss of water to the external ice which may cause the damage of the cell. In the year of 1950s they are rapid development of the freezing techniques which made helping in bringing the pregnancies. Before this the insemination of frozen sperm was brought into live. Latter in the 1957 the scientist of the United Kingdom started the cryopreserving the fowl sperm. In the year of 2000s the baby was born by the cryopreservation egg, Laina Beasley born in July 2005. Not only in the human beings, this is brought into the animals which made to the result of A Ocelots kitten born in Cincinnati Zoo in 2001. As freezing damage in the cells are of two aspects. The primary one is that cell gets damage due to the ice crystal, and the second is the damage of cell when more ice is formed due to the concentrate of the solute. Latter in the USA they made a solution for this aspects of the damage in cell by the typical rate of cooling 1C/min but this rate of cooling depends on the size of the cell and the water content in the cell. In this they are a form of anti-freeze known as the cryprotectant which is used to equalize the physical optimal parameter osmotic. Cryoprotectants have ability to protect the cell to face the freezing injury which was discovered accidentally. Cryoprotectants: When the biological materials are kept under the preservation they are need to be protect for the long time. At same time the protected material should be able to function for a long time when they are rewarmed to the sub zero level. During the process of the preservation some chemicals are used to preserve them in low temperature and in the same way they are rewarmed, and should have the ability to function for a long time. But in some cases of preservation chemicals are not used such as in fungi, yeast. The cryoprotectants are used in this cases, now a days some chemicals like dimethyl sulfoxide, glycerol. But in some of the specimens the dimethyl sulfoxide affects the preservation due to the toxicity nature. (Smith, 1983) This toxicity can be reduces to some level by use of glucose Advantages of Cryoprotectants: Helps the material from rapid cooling Prevents from formation of ice in the intracellular region. When the cell undergoes high concentration of solute it helps to prevent from dehydration (Mazur, 1984). Helps the cell to function even after the rewarming. Slow cooling: This is the early technique used in the cryopreservation which is used to prevent to the cell from the damage in the freezing Background: It is the control rate technique which was developed in the 1970s which has been enabled the first human embryo birth. From then this technique is used all over the world for the biological materials. And some machines which are used in the cryopreservation bring the cell to the freezing point such as the liquid phase of the liquid nitrogen. This technique machines are used to freeze the oocyte, blood products, sperms, skin, embryo, general tissues and stem cells preservation in research labs, hospitals all over the world. But in the slow cooling the cell gets dehydrate Vitrification: This is the new technique used in the cryopreservation which is used to prevent to the cell from the damage in the freezing. It is the preservation at extremely low temperature without any freezing. In this process can be done without the involvement of the cryoprotectants. Background: Right from the development of the slow cooling the glycerol is used to cryobiology as the cryoprotectant for the bull sperm and blood cells. But however it is know that glycerol is not helpful to prevent the whole organ from the damage. For more suitable cryoprotectants in those cases many of the biotech companies worked to develop. In the 21st century the kidney of rabbit is preserved at -135oC, which made as the vitrification cocktail, because latter the kidney which is preserved at the -135oC was again planted back into the body of rabbit, the kidney was found to be functioning without any failure. At present preservation of the brain is under the progress, they are looking to prevent the brain from damaging such as damage to the tissues and loss of the memory in the brain which was encoded. The Institute of Cryonics are working to preserve the whole body without damage in the cells, tissue and all the organs which should again function properly when they are transplanted, this is in the progress. In this the freezing involves in ice crystal formation, which lead to the damage of the sensitive structures such as the blood vessels. For a successful vitrification it needs combinations of the two factors, one is the high concentration of solutes in the bathing medium capable of glass formation, and the other is the extreme rapid cooling of the samples. In the year 1985 for the first time the cryopreservation of mouse embryos by Rall and Fahy. Steps that to be followed for the successful vitrification are concentration and composition of the vitrification solution The procedure used to equilibrate cells in this solution The cooling/warming conditions The procedure used to dilute cells from the vitrification solution Freezing injuries: In a living cell the liquid water is most important to maintain its structure and function, when this cell is kept in the freezing preservation, due to the low temperature then to its survival then the cell faces the freezing injuries which may lead the living cell to destruction. When the cell is under the preservation the injury that effect is shown in the figure the inverted U in this the position of the cell which it can function normally is shown as the survival point , when this cell is put on to the freezing beyond its limit, that is a cell has its own capability for a certain limit of low temperature or high temperature, when this cell exceeds the limit of low temperature the solution around the cell makes it injury, in such cases the intracellular ice formation will be occurred, at this stage the cell leads to the injury and destruction occurs. In some cases like the high cooling rate the cell undergoes both the extracellular and intracellular. Freezing injuries at high cooling rate: When we take most of the cells they have the thermodynamic point at -0.5oC. But when we need to preserve the cell the cell must get freeze, to do this the cell will be undertaken below -5oC. At this position the cell undergoes the super cooling at which the medium around the cell and the cell remain unfrozen, due to the protective solute that is bounded around and within the cell. The cell which is taken to the low temperature between the -5oC and -15oC the ice forms in the external medium. At which the cell content remain super cooling in an unfrozen state. The ice which is formed in the external medium will affect the extracellular solute. The solution concentration in the extracellular solution will increase when the temperature gets decreases and the ice will be grown, this increase of ice is the ice phase. Due to this the chemical imbalance is occurred between the biological material and the unfrozen external solution. The external part of the cell gets frozen when the water flows off, this occurs when the higher chemical potential then the water of the partly frozen solution outside the cell. And this subsequent physical event in the cell depends on the rate of cooling in the cell. If the cooling is sufficiently slow, the loss of water rapidly by exosmosis. When this occurs the result of the cell will dehydrate and will not freeze intracellular. TZ p3 When the cooling is too rapid the rate at which the chemical potential of water extracellular solution decreases is much faster than to the rate which water can be diffuse out of the cell and they will be the end result in the intracellular ice formation. In the shown figure the cell under the preservation will have the outflow of the intracellular water which may lead to shrink of the cell and the extracellular ice will be formed which leads to the shrunken cell with little or no ice formed internally. It is the indirect assumption that the formation of the ice inside the cell is unpreventable. At present many of the studies have been suggested that intracellular ice formation during the process of the freezing causes the death or damage of the cell. In the process of the intracellular ice formation they are three possible ways which it can be occurred. Chilling injuries: Chilling injury is defined as the low temperature stress on the absence of freezing. Actually the word chilling injuries is used in the botany, in the early 18th centuries to describe the plants which are subjected to the low temperature that is chilling temperature above the 0Â °C were often damaged irreversibly. The temperature shock was first used in 1934 to show the irreversibly damage to mammalian sperms that occurred when these cell undergo rapid cooling below the body temperature at which few degree fall down rapidly in a minute of time. At these both sperm cells and the plant cells the chilling injury are similarly related mechanism. In the process of chilling injuries they are two types direct chilling injury and the indirect chilling injury. Direct chilling injuries: This is also known as the Cold shock. This is mostly used to describe both phenomena, which is expressed quickly upon reduction in temperature and Dependent on cooling rate. Cold shock injury is almost independent of the rate of warming. Injury is increased as the period incubation at the reduced temperature is extended. Indirect chilling injuries: Indirect chilling injuries are usually evident following a relatively long exposure period at the time of the reduced temperatures, and its enable to the independent of the rate of cooling. Metabolic and enzymatic processes can find in the fast developing embryos. Especially in Drosophila and zebrafish the injury get more rapid at the low temperatures. This is due to the co-ordination is increased lost with decreasing temperature. The reduction in temperature will affect the enzyme rate reaction to a different extent. SIMILARITIES BETWEEN CONTROLLED SLOW COOLING AND VITRIFICATION: (Baudot et al., 2002) In the process of preservation both the techniques have the similarity of freezing during the process of preservation. In slow cooling the cooling is done intracellular and extracellular and in the same way in vitrification, but little change at place where ice crystal formation is occurred in slow cooling and not in vitrification Somehow both techniques are similar with slight changes during the process of the preservation of biological materials. DIFFERENCES BETWEEN CONTROLLED SLOW COOLING AND VITRIFICATION IN CRYOPRESERVATION OF BIOLOGICAL MATERIALS: (Kuleshova, 2002) Vitrification techinque Slow cooling technique This is simple technique This is complex technique This safer technique This is risky technique This more costly technique This cheaper compare to vitrification Ice crystal dont form in the process of freezing In this ice crystals formation is seen This is most successful technique Not much success then vitrification Cell death will not occur Have the chances to the cell death ADVANTAGES OF VITRIFICATION AND CONTROLLED SLOW COOLING IN CRYOPRESERVATION OF BIOLOGICAL MATERIALS: In the cryopreservation the both techniques vitrification and controlled cooling techniques are used to preserve the biological materials for a long time. Vitrification technique has the uniqueness for the preservation of the oocytes, because the oocytes brought under this technique have more capable to the fertilization. This oocytes lead to the normal pregnancy. In process of the vitrification the ice crystal formation is not occurred both in the intracellular and the extracellular. In vitrification the whole cell including the medium solidify (freeze). In the process of vitrification the cell doesnt get any damage and dont lead the cell to death (Kasa, 2004). The preservation of materials at a controlled slow cooling, we can store the materials at -196oC, best example is storage of hematopoietic cells (Hill et al., 1972). The main advantages of cooling and warming rates are that it contains very less amount of cryoprotectants, with this it can reduce toxic effect and also osmotic injury (Orief et al., 2005). DISADVANTAGES OF CONTROLLED SLOW COOLING AND VITRIFICATION IN CRYOPRESERVATION OF BIOLOGICAL MATERIALS: When we come to the vitrification we dont face any unfavarable conditions during the process of preservation, because of cryoprotectants which toxic in nature and more cost(Chi, 2001). Ice crystals are occurred in the intracellular and the extra cellular region of cell in the process of preservation in slow cooling technique. This is the major disadvantage in controlled slow cooling. (Kasa, 2004). Main Outcome Measure: As per the reported number of pregnancies done after transfer of embryos which were cryopreserved by vitrification. Both slow cooling and vitrification procedures have successful cryopreservation of human embryos and oocytes. Both procedures have healthy births, but slow cooling of oocytes gives very low success rates. Vitrification is a promising novel technique in reproductive technology CONCLUSION: As per the reference and my knowledge controlled slow cooling and as well as vitrification are useful techniques for the preservation of biological materials, when compared vitrification technique is more useful technique for the preservation as slow cooling technique. Vitrification is a simple procedure that requires less time, safer and more cost effective than slow cooling.